منابع مشابه
Isolation of Adipose Tissue Stem Cells with Organ Culture Method
Background Isolation of adipose tissue mesenchymal stem cells (MSCs) with current enzymatic methods has some limitations. For example, it is costly and time-consuming and results in a heterogeneous cell population that making compromise proliferation and differentiation of MSCs. Also, it is accompanied with the increased risk of culture contaminations because of several handling steps.In this...
متن کاملisolation of adipose tissue stem cells with organ culture method
background isolation of adipose tissue mesenchymal stem cells (mscs) with current enzymatic methods has some limitations. for example, it is costly and time-consuming and results in a heterogeneous cell population that making compromise proliferation and differentiation of mscs. also, it is accompanied with the increased risk of culture contaminations because of several handling steps.in this ...
متن کاملPrimary culture of porcine pancreatic acinar cells.
INTRODUCTION The methodology of acinar cell culture has become of primary importance in the research of pancreatic physiology and pharmacology. AIM To develop a method for primary culture of porcine pancreatic acinar cells. METHODOLOGY Dispersed pancreatic acinar cells were made by RPMI-1640 medium containing collagenase III. After purification, the isolated acinar cells were cultured in RP...
متن کاملIntegration of embryonic stem cells in metanephric kidney organ culture.
Many stages of nephrogenesis can be studied using cultured embryonic kidneys, but there is no efficient technique available to readily knockdown or overexpress transgenes for rapid evaluation of resulting phenotypes. Embryonic stem (ES) cells have unlimited developmental potential and can be manipulated at the molecular genetic level by a variety of methods. The aim of this study was to determi...
متن کاملMicrotubules containing acetylated alpha-tubulin in mammalian cells in culture
The subcellular distribution of microtubules containing acetylated alpha-tubulin in mammalian cells in culture was analyzed with 6-11B-1, a monoclonal antibody specific for acetylated alpha-tubulin. Cultures of 3T3, HeLa, and PtK2 cells were grown on coverslips and observed by immunofluorescence microscopy after double-staining by 6-11B-1 and B-5-1-2, a monoclonal antibody specific for all alph...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the Japan Academy
سال: 1967
ISSN: 0021-4280
DOI: 10.2183/pjab1945.43.556